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KMID : 0379120100380020152
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Korean Journal of Mycology
2010 Volume.38 No. 2 p.152 ~ p.159
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Purification and Characterization of Urushiol Induced Laccase Isoenzyme from Fomitella fraxinea
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Choi Han-Seok
Park Hyo-Suk
Yeo Soo-Hwan
Jeong Seok-Tae
Choi Ji-Ho
Kim Myung-Gon
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Abstract
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The influence of urushiol, as an allergen on laccase property of Fomitella fraxinea was investigated. The enzyme production was reached to the highest level after 10 days, cultivation and the activity and mycelial biomass were increased by 2.5 and 1.5 folds, respectively, by adding urushiol in the culture medium. In liquid cultures using a Cu Mn-free medium, laccase lactivity was decreased by 3.8-9.2 folds, with similar dry cell weight. Two isoenzymes, were purified using anion exchange, hydrophobic interaction and size-exclusion chromatographies. Both isoenzymes are monomeric proteins, with around 67 kDa(Lac1) and 66 kDa(Lac2), and isoelectric points of 3.67 and 3.81. The optimal conditions for purified isoenzymes were found to be pH 4.5-5.0 and . Activity decreased by the addition of , , , and strongly inhibited by EDTA and sodium azide.
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KEYWORD
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Basidiomycetes, Fomitella fraxinea, Laccase, Purification, Urushiol
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